Table 1
Characterization of isolated bacterial strains inducing mineral agglomerates on the surface of the colonies.
| Name code | Isolation sample | Isolation and culture media | Morphotype | Respiratory type | Motility | pH of culture media |
|---|---|---|---|---|---|---|
| CD1 | Calcareous deposits | MB + CaCl2 | Gram-negative bacilli | FA | Motile | 8.1 ± 0.1 |
| CD2 | Calcareous deposits | MB + CaCl2 | Gram-positive bacilli | FA | Motile | 8.1 ± 0.2 |
| CD3 | Calcareous deposits | MB + CaCl2 | Gram-negative bacilli | FA | Motile | 8.1 ± 0.1 |
| CD4 | Calcareous deposits | MB + CaCl2 | Gram-negative bacilli | FA | Motile | 8.1 ± 0.2 |
| CD5 | Calcareous deposits | MB + CaCl2 | Gram-negative bacilli | FA | Motile | 8.0 ± 0.2 |
| CD6 | Calcareous deposits | MB + CaCl2 | Gram-positive bacilli | FA | Motile | 7.9 ± 0.1 |
| CD7 | Calcareous deposits | MB + CaCl2 | Gram-positive bacilli | FA | Non-motile | 8.1 ± 0.2 |
| CD8 | Calcareous deposits | MB + CaCl2 | Gram-positive cocci | FA | Non-motile | 8.2 ± 0.2 |
| CD9 | Calcareous deposits | M1 + CaCl2 | Gram-negative bacilli | FA | Motile | 7.9 ± 0.1 |
| CD10 | Calcareous deposits | M1 + CaCl2 | Gram-negative bacilli | FA | Motile | 7.9 ± 0.1 |
| Md1 | Mud near of calcareous deposits | M1 + CaCl2 | Gram-negative bacilli | FA | Motile | 8.0 ± 0.2 |
| SW1 | Sea water of La Rochelle site | M1 + CaCl2 | Gram-negative bacilli | OA | Motile | 8.0 ± 0.1 |
| SW2 | Sea water from Angoulins site | M1 + CaCl2 | Gram-negative bacilli | FA | Motile | 8.0 ± 0.2 |
| SW3 | Sea water from Angoulins site | M1 + CaCl2 | Gram-negative bacilli | OA | Motile | 7.9 ± 0.1 |
| Sporosarcina pasteurii DSM 33* + 2% urea | Isolated from soil | M220 + CaCl2 | Gram-positive bacilli | FA | Motile | 9.1 ± 0.1 |
Marine bacteria grew for 3 days on solid media at 25 °C. *Sporosarcina pasteurii DSM 33 was used as a model bacterium for its ability to biomineralize [23]. FA: facultative anaerobe; OA: obligate aerobe.
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